Abandoned by her mother years ago, Opal (AnnaSophia Robb), a 10-year-old girl, moves with her preacher father (Jeff Daniels) to a small town in Florida. Lonely and missing her old friends, Opal is ecstatic to find companionship with a rambunctious little dog she names Winn-Dixie, after the store where she found the pup. With Winn-Dixie's help, Opal befriends several colorful townsfolk (Cicely Tyson, Eva Marie Saint, Dave Matthews) and even begins mending her relationship with her father.